FuGENE® HD Transfection Reagent(a) is a novel, nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity.
What is FuGENE transfection reagent?
FuGENE® HD Transfection Reagent is a multi-component reagent that forms a complex with DNA, then transports the complex into animal or insect cells. It is suitable for use in media with or without serum, and for transient or stable transfection, as well as co-transfections of multiple DNA plasmids.
What is lipofectamine transfection?
Lipofectamine™ Transfection Reagent is a value alternative for transfection of plasmid DNA into eukaryotic cells with a simplified protocol. For optimal results in a wider range of plasmid DNA transfection conditions, we recommend Lipofectamine™ LTX Reagent, due to its optimal balance of potency & low-cytotoxicity.
Is FuGENE toxic to cells?
The FuGENE® 6 and FuGENE® HD Transfection Reagents are nonliposomal reagents that transfect DNA into a wide variety of cell types with high efficiency and low toxicity.
What does FuGENE do? – Related Questions
Is FuGENE better than Lipofectamine?
FuGENE® 4K reagent outperforms Lipofectamine® 3000.
Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % GFP expressing cells and % total cell viability (via propidium iodide dye). The graphs above show the superior performance of FuGENE® 4K vs. Lipofectamine® 3000.
Is FuGENE lipid based?
The FuGENE HD Transfection Reagent is lipid-based, simple-to-use, and can result in high transfection efficiencies with minimal cyototoxicity.
Is Lipofectamine toxic to cells?
The transfection efficacy of Lipofectamine 2000 was compromised by its high toxicity, which may adversely affect its application in most cells.
Is transfection toxic to cells?
Transfection of cells at lower densities often leads to cellular toxicity because of an increase in the number of complexes delivered per cell.
What does Lipofectamine do to cells?
Lipofectamine or Lipofectamine 2000 is a common transfection reagent, produced and sold by Invitrogen, used in molecular and cellular biology. It is used to increase the transfection efficiency of RNA (including mRNA and siRNA) or plasmid DNA into in vitro cell cultures by lipofection.
Are HEK cells cancerous?
HEK 293 can be employed as a highly malignant tumor model as its tumorigenicity increases significantly in high-passage. The tumorigenicity of the HEK 293 cell line reached 100% when the passage exceeded 65. 1.
Who did HEK293 come from?
What Is HEK293? HEK293 are immortalized human embryonic kidney cells. Originally isolated in the 1970s by Alex Van der Eb, a Dutch biologist, it was postdoc Frank Graham who transformed the cell line with sheared adenovirus 5 (Ad5). The ‘293’ refers to the fact that it was Graham’s 293rd experiment.
Where did HEK293 come from?
The human embryonic kidney or HEK 293 cell line was derived from human embryonic kidney cells grown in tissue culture. The cell line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.
What else is HEK293 used for?
HEK293 is commonly used in the production of viral vectors. It is used in the production of adenoviral and adeno-associated viral vectors due to presence of the adenoviral E1A/B genes which provide helper functions during viral vector production.
Are HEK293 immortal?
Thus, HEK293 is not a model for normal human cells; these cells are immortalized already by known oncogene but not malignant yet.
Are HEK cells female?
Originating from a female fetus, HEK293 cells are today among the most used mammalian cell lines for a wide range of applications because of their ease of transfection as well as culture.
Are HEK cells safe?
According to the CDC, the HEK293 cell line is Biosafety level 2. BPS recommends checking with your local federal, state, and institutional regulations. If in doubt, we recommend using the highest appropriate biosafety level requirements.
How fast do HEK293 cells grow?
HEK-293 cells see cell doubling once every 24–48 hours depending on the care. Most HEK-293T cells double every 12–20 hours depending on conditions. Our cell doubling time is typically closer to 12 hours.
Is HEK293 cloned?
HEK293FT cells were cloned from the HEK293T cell line and adapted to commercial media. HEK293FT is designed for lentiviral production. Similar to HEK293T, the 293FT cells stably express the SV40 large T antigen from the pCMVSPORT6TAg.
